Observatorio de Investigación y Desarrollo Tecnológico


  • REVISTA CUBANA DE FARMACIA//(VOL. 52, NUM. 4)

    Volumen: 52, Numero: 4, Páginas: [16]p.

    COMPOSICION Y ACTIVIDAD ANTIBACTERIANA DEL ACEITE ESENCIAL DE AUSTROEUPATORIUM INULIFOLIUM (KUNTH) KING & ROBINSON (ASTERACEAE)

    Abstract

    Introduction: Austroeupatorium inulifolium (Kunth) R.M.King and H.Rob. (Asteraceae) are species of the Asteraceae family that grow in South America, from Panama to Argentina. Objective: To determine the composition and antibacterial activity of the essential oil of Austroeupatorium inulifolium (Kunth) King and Robinson (Asteraceae) on Staphylococcus aureus, Enterococcus fecalis, Escherichia coli, Klebsiella pneumoniae, and Pseudomona aeruginosa.Methods: The fresh leaves of Austroeupatorium inulifolium were harvested in January 2018 in via San Pedro, in the parish Tovar, Tovar Municipality, Mérida state, Bolivarian Republic of Venezuela. The fresh leaves were liquefied and subjected to distillation by dragging with water vapor (3 h), using a trap of Clevenger. The oil was retained to - 4 °C until its use for biological tests. The essential components of the oil were analyzed by gas chromatography coupled to mass spectrometry. The antibacterial activity was evaluated according to the agar diffusion method with paper disks.Results: The performance of the oil obtained by hydrodistillation was 0.6 percent. There were identified 35 compounds. The main components were: ?-pinene (6.94 percent), ?-pinene (9.25 percent), ?-caryophyllene (13.65 percent) and germacrene D (21.12 percent).Conclusions: The essential oil of A. inulifolium presents low activity against gram-positive bacteria: Staphylococcus aureus (CIM 5000 ?g/mL) and Enterococcus faecalis (10 000 ?g/mL), while it presents good activity against gram-negative bacteria with a MIC of 78 ?g/mL for Escherichia coli, Klebsiella pneumoniae and Pseudomona aeruginosa.


    Keywords


    Asteraceae, Austroeupatorium inulifolium, essential oil, germacrene D, ?-caryophyllene, ?-pinene, ?-pinene, antibacterial activity


    Resumen

    Introducción: Austroeupatorium inulifolium (Kunth) R.M.King


    Palabras Clave


    Asteraceae, Austroeupatorium inulifolium, aceite esencial, germacreno D, ?-cariofileno, ?-pineno; ?-pineno, actividad antibacteriana





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