Volumen: 3, Numero: 1, Páginas: p.
The essential oil of the leaves from Piper amalago L. (Piperaceae) obtained through hydrodistillation was analyzed by gas chromatography and gas chromatography-mass spectroscopy (GC/MS). The antibacterial and antifungal activities of the volatile oil was evaluated by the broth microdilution technique and was tested against Gram positive (Staphylococcus aureus and Enterococcus faecalis) and Gram negative (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) bacteria, and the yeast Candida albicans. Altogether 38 compounds (90.16 percent) were identified in the sample and the major constituents found were ?-phellandrene (20.42 percent), spathulenol (10.34 percent), bicyclogermacrene (8.50 percent) and ?-pinene (7.29 percent). The essential oil howed a moderate antibacterial effect against S. aureus and E. faecalis (MIC 100-200 ?g/mL). Additionally, E. coli was the Gram negative strain resulting be most susceptible (MIC 50-200 ?g/mL), since the oil had little ability to inhibit the growth of P. aeruginosa and K. pneumoniae (MIC > 1000 ?g/mL). A weak antifungal efect was showed against C. albicans. These results suggest that the P. amalago essential oil may be used as antimicrobial agent in biotechnological processes employed in food, pharmaceutical and other industries. According to the literature consulted, this is the first report on the antimicrobial activity of the essential oil of P. amalago from Ecuador.